Anti Lamin Antibody What Is It

These proteins are found in many different cell types in three different forms a b and c.

Anti lamin antibody what is it. This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. However based on bioassays it appears that the mix of antibodies in this purified polyclonal anti serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited. Fisher pa journal of cell science 108 pt 9. Goat anti rabbit igg h l hrp preadsorbed at 1 5000 dilution developed using the ecl technique.

These proteins are found in many different cell types in three different forms a b and c. Species predicted to react based on 100 sequence homology. It is the best among many laminin function blocking preps we have tested. Expression of drosophila lamin c is developmentally regulated.

Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. Weber k journal of cell science 108 pt 10. Analogies with vertebrate a type lamins. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda.

Anti lamin antibodies are available from several suppliers. Anti lamin antibody products lamin is a reported alias of the human protein lamin a c encoded by the gene lmna. Lamin a c antibodies lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.

Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Anti lamin a antibody ab26300 at 1 µg ml lane 1. Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin. Site mapping using a monoclonal antibody.

Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin. Recombinant human lamin a protein at 0 01 µg secondary all lanes. Recombinant human lamin a protein at 0 1 µg lane 2. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.

Interphase phosphorylation of the drosophila nuclear lamin. Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa. Performed under reducing conditions.